Three commonly used extraction solvents (70% ethanol, 80% acetone and acidic 70% acetone) were evaluated for
yields of phenolic substances and subsequent antioxidant capacity of the extracts from three samples (raw, dried and
autoclaved) of African oil bean (Pentaclethra Macrophylla Benth) seed in a 3x3x4 factorial design. The contents of
selected phytochemicals, namely, total phenolic content (TPC), total flavonoid content (TFC) and condensed tannin
content (CTC) were investigated. In addition, antioxidant activities were tested using 2,2-diphenyl-1-picryphydrazyl
(DPPH). The results show that for extraction, 70% ethanol gave more yields of extracts from raw and dried samples,
while 80% acetone gave more yield of extract from the autoclaved sample. Moreover, the acidic 70% acetone gave the
highest yield among the three solvents for extracting TPC from the three samples while for the TFC 80% acetone gave
the highest yield. The best extraction solvent for DPPH antioxidant assay was 70% ethanol.
Key words: Pentaclethra macrophylla, seed extract, heat treatment, extraction solvent, DPPH
PAPER TITLE :ANTIOXIDANT PROPERTIES OF AFRICAN OIL BEAN (PENTACLETHRA MACROPHYLLA BENTH) SEED PHENOLICS AS INFLUENCED BY EXTRACTION SOLVENTS AND HEAT TREATMENTS
APPLIED TROPICAL AGRICULTURE | VOLUME 24 NUMBER 1 2019
Paper Details
- Author(s) : Oyinloye , A. M.* and Enujiugha, V. N.
- Abstract:
