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Pycnanthus angolensis is an important multi-medicinal tropical tree species that is vulnerable owing to high rate deforestation and exploitation without replacement. A study was carried out to develop a macropropagation protocol using stem cuttings and to investigate the effects of plant growth regulator IBA on rooting of both mature tree and juvenile stem cuttings of P. angolensis. Stem cuttings were collected from both the mature tree in situ and young seedlings within the period of May to August 2017. Stem cuttings were treated with Indole Butyric acid (IBA).The control cuttings were dipped in sterile water. Only cuttings from juvenile seedlings responded to treatment with IBA. The result revealed a significant effect of IBA concentration on the rooting performance of the stem cuttings and number of shoots per cutting. The highest rooting percent of cuttings from juvenile seedlings was recorded with 2000ppm IBA which was significantly different (P< 0.05) from 2500ppm IBA. After acclimatization, maximum survival rate of plants was 70%. A successful macro propagation protocol has been developed; therefore, it can be employed for uniform plantation establishment of P. angolensis for mass propagation of this multi-medicinal tree species and tree planting programme.

Keywords: Macropropagation, Juvenile Stem cuttings, P. angolensis