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Iodine deficiency disorder (IDD) is a public health problem in developing countries such as Nigeria and universal salt iodization has been chosen to be the basic measure for sustainable elimination of IDD. This study evaluated the knowledge, attitude and practice of mothers regarding iodized salt in selected communities in Ibadan. The study was carried out among 250 purposively selected mothers of under-5 children in three randomly selected local government area of Ibadan, Oyo state. Nigeria. A structured interview schedule was used to obtain relevant information from the respondents. Data were analyzed using Statistical Package for Social Sciences version 15. Level of significance was 5%. Chi square test was used to test for the relationship between variables. Less than a third (22.1%) had less than secondary education. About52%were either full housewives or petty traders. Seventy nine percent have heard about iodized salt but only 48.4% know the main sources of iodine was iodized salt while 51.6% had inadequate knowledge about the sources of iodized salt. Most of the mother (58.0%) had positive attitude towards iodized salt. More than half of the mothers (53.2%) had poor practices regarding iodized salt. Knowledge about iodized salt was found to be significantly associated with usage of iodized. There was association between the attitude and consumption of iodized. The study showed that most mothers with children under 5 years in Ibadan had positive attitude towards iodized salt, however, their knowledge and practices were inadequate.