PAPER TITLE :IN VITRO CONTROL ON FUNGUS ASSOCIATED WITH BIO – DETERIORATION OF SWEET POTATO (IPOMOEA BATATAS (L.) LAM) TUBERS

FUTA JOURNAL OF RESEARCH IN SCIENCE | VOLUME 9 NUMBER 1 2013

Paper Details

  • Author(s) : M.N. Suleiman* and T.N. Falaiye
  • Abstract:

Apparently healthy and rotted sweet potato tubers (Ipomoea batatas (Linn.) Lam) were obtained from selected
markets and farms from Anyigba, Egume, Ejule and Dekina, Dekina Local Government Area, Kogi State,
Nigeria. Isolation and identification of fungi associated with rotten sweet potato tuber was carried out. Diseased
portion of the rotted tubers were scraped and inoculated on Potato dextrose agar (PDA) at 27±20C, sub-cultured
to obtain pure culture. Aspergillus niger was isolated from the rotten portion of the tubers and identified based
on morphological characters and pathogenicity tests. Leaf aqueous extracts of neem (Azadirachta indica (Linn.)
and bitter leaf (Vernonia amygdalina) were evaluated for their inhibitions at concentrations of 10%, 20%, 30%
and 40% on the isolated fungus. The study showed that neem leaf extract was effective in suppressing mycelial
growth of A. niger at higher concentration (30% and 40%); which could be employed in the biocontrol of
infections caused by A. niger. The percentage inhibition at their various concentrations was also determined.
Key words: Sweet potato, tubers, Aspergillus niger, Extract, rotten, Vernonia amygdalina, Azadirachta indica