PAPER TITLE :PROTECTIVE ACTIVITY OF ACETYLSALICYLIC ACID ON SELECTED TISSUES OF ALBINO RATS TREATED WITH POTASSIUM BROMATE.

FUTA JOURNAL OF RESEARCH IN SCIENCE | VOLUME 10 NUMBER 2 2014

Paper Details

  • Author(s) : 1J.E. Olajide, 2.M.A. Akanji, 1.M. Sanni, 1D.O. Ejembi and 1O.C. Abbah
  • Abstract:

The protective role of acetylsalicylic acid on potassium bromate-induced toxicity in rat tissues was investigated.
Twenty male albino rats of average weight, 200+4.5g were grouped randomly into four groups of five rats per
group. Group 1 received distilled water only and served as the control. Group 2 received 1 ml solution containing
20mg/kg body weight of potassium bromate (KBrO3). Group 3 received 1ml solution containing 20mg/kg body
weight of acetylsalicylic acid (ASA). And group 4 received 1 ml solution of 20 mg/kg body weight of KBrO3
and ASA concurrently. Distilled water and the solution of chemical substances were administered to rats orally
for 21 days. Physical observations of the rats administered with potassium bromate include rapid breathing,
impaired locomotion and diarrhoea. Enzymes assay revealed significant reductions (P<0.05) in activities of
major marker enzymes-alkaline phosphatase, (ALP); Lactate dehydrogenases, (LDH) and glutamate
dehydrogenase, (GDH) in the tissues followed by corresponding significance increases (P<0.05) in activities of
these enzymes in the serum upon administration of solution of KBrO3. Acid phosphatase activity was not
significantly (P>0.05) affected. When ASA alone was administered and enzyme activities were compared with
both the control and the KBrO3 group, it was discovered that activity values in the tissues and serum tend
towards the control as well as being significantly higher (P<0.05) than the values obtained when KBrO3was
administered. However, concurrent administration of KBrO3 and ASA solutions to the rats, nullified the effects
of KBrO3 on these enzymes activities rather the activity values were elevated with no significant difference
(P>0.05) compared to the control but significantly higher (P<0.05) when compared to the administration of
KBrO3 alone. The observed trends in enzymes activities following administration of KBrO3 suggested damage to
the plasma membrane of cells. The trend however changed when solutions of KBrO3 and ASA were
administered concurrently indicating a probable ability of ASA to prevent the disruption of the ordered
membrane structure of the cells. The dose of acetylsalicylic acid that was employed in this work was able to
prevent enzyme leakage through the membrane of the cells and it is the likely attribute of acetylsalicylic acid as a
membrane stabilizer.
Keywords: Potassium bromate, acetylsalicylic acid, liver, kidney