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The search for new antibiotics remains a priority in the face of challenges posed by increasing antimicrobial resistance among bacterial pathogens. Soil samples were collected from five specific biotopes including rhizosphere, poultry, compost, earthen fish pond and stockyard respectively, in Igodan, Okitipupa Local Government Area of Ondo State. They were analyzed for potential antibiotic producing Bacillus and Streptomyces species of bacteria. Aliquots of 10-4 and   10-5 dilutions of soil samples were inoculated on Nutrient agar and modified Streptomyces Isolation agar (MSIA). Bacillus isolates were identified using colonial characteristics, Gram-staining and biochemical tests, while Streptomyces and molds were identified by appearance of aerial mycelium. Ability of Bacillus species to produce antibiotics was tested by their inhibitory effects against five target organisms. The isolates consisted of five Bacillus strains (41.7%), three Streptomyces strains (25%) and four Penicillium strains (33.3%). On MSIA, Streptomyces coelicolor strains PgsS1 and RzsS4 were isolated from stockyard and rhizosphere. Incorporation of 250,000 IU of nystatin antifungal per liter of MSIA did not inhibit the growth of Penicillium strains growing on the medium. However, 2500 mg/L of rifampicin added to MSIA as an antibacterial completely inhibited all bacterial growth. The findings of the present study reveal abundance of potential antibiotic producers in different biotopes in the locality under study.