The binding interaction of kojic acid with β-amylase was studied under simulative conditions using fluorescence spectroscopy. The results obtained revealed kojic acid quenched intrinsic fluorescence of β-amylase to form βamylase-kojic acid complex. The quenching was a static quenching with effective quenching constant (Ksv) of 2.13 X 102 L. mol-1 . The Scatchard plot indicated that β-amylase has one binding site for Kojic acid with association constant of 1.55 x 103 L. mol-1 at 298 K and pH 7.4. The bonding represents a favourable bonding interaction. With these, β-amylase as a nano-vehicle for kojic acid is hereby suggested.
Keywords: Kojic acid; β-amylase; Fluorescence; Quenching.
