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African locust bean effluent contains a range of microbes and has been found to be of health benefit to human. The present study aims to identify various microorganisms associated with African locust beans effluents and also determine its phytochemical parameters. Cooked locust beans under hygienic condition in the laboratory containing the effluent with chaffs (EFWS) and the effluent without the chaffs (EFWOS) were used in this study. The conventional and molecular methods of identification of microorganisms were employed in the identification of microbial isolates of the effluent. Quantitative and qualitative analyses were used to determine phytochemical components in the effluents using standard methods. Bacillus subtilis, Enterobater faecalis, Enterobacter aerogenes, Acaligenes faecalis, Lactobacillus sp., Aspergillus niger, Aspergillus flavus, Penicillium, Rhizopus and Candida species were identified using conventional method. The molecular techniques revealed that the bacteria had (98-100%) homology with Bacillus subtilis 8700:2, Lactobacillus paracasei D12-5 and Enterobacter ludwigii TB-136 while the fungi had remote identity (88-98%) with Aspergillus flavus Z5, Geotrichum candidum OUCMBI101144 and Trichomonacaea sp. LMI. Predominant microorganisms isolated were Bacillus subtilis and Aspergillus flavus. The phytochemical components of the effluents were saponin, tannin, flavonoid, terpernoid, and alkaloid. Saponin had the highest concentration with 39.82 mg/g and 39.27 mg/g for EFWS and EFWOS respectively while tannin had the lowest concentration with 4.17 mg/g for the effluents. This study has provided additional and useful information on the diversity of microorganisms and the phytochemical constituents associated with the effluents of Parkia biglobosa for those interested in consuming the effluents for therapeutic purpose.

Keywords: Locust beans, Polymerase Chain Reaction, Microorganisms, Phytochemical,