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One of the world medical problems is the challenge of bacteria and fungi resistance to commercial antibiotics. Recently some organisms such as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, and Enterococcus faecalis to mention but a few have developed drug resistance. This study aimed to quantitatively determine the phytochemical constituents of Raufolvia vomitoria seed, leaf and root. It further aimed to evaluate the antimicrobial effect of the ethanolic extract of seed, leaf and root against some selected bacteria (Bacillus subtilis, Bacillus cereus, Pseudomonas syringine, Pseudomonas aeurginosa, Escherichia coli, Xanthomonas axonopodis and Staphylococcus aurus) and fungi (Cyrtomium falcutum, Rhizoctonia solani, Colletotrihum lindematianum, Trichophyton rubrum). Phytochemical analysis was determined using standard procedures and antimicrobial activity was performed by agar well diffusion. The result revealed that the root had the highest alkaloids, phenols,phytates and saponins contents of 15.72 %, 1.24%, 1.66% and 5.99% respectively. The seed had the highest flavonoid contents of 9.38 and the leaf recorded the highest oxalates content of 0.17 %. The result revealed that all the ethanolic extracts are potent against the test microbe. Inhibition zones of the extract against bacteria ranged from 1.20 mm to 10.00 mm while the mycelia inhibition against fungi ranged from 4.76 mm to 26.98 mm. The research demonstrates that extracts from the seed, leaf and root of Raufolvia vomitoria could be used for production of broad pharmaceutical and medicinal based products.  
 
Keywords: Rauvolfia vomitoria, phytochemical, bacteria, fungi, Extract and Medicinal