Contaminated water bodies arising from oil exploration and production activities
pose serious threats to aquatic life. In the present study, juvenile African catfish (Clarias gariepinus)
were exposed to lethal concentration of crude oil (0.9 %, v/v) and bioaugmentation treatment using
10% mixed culture of Alcaligenes eutrophus (BNS-09) and Bacillus sphaericus (BNS-05) for 96
h compared with control. Life sustaining indices (pH, dissolved oxygen (DO) and conductivity were
monitored. Markers of oxidative stress (superoxide dismutase, catalase, reduced glutathione and
lipid peroxidation (malondialdehyde) levels) were evaluated in catfish liver. Results showed
significant decrease in pH and DO with increase in conductivity in crude oil polluted water (CPW)
compared with control. There was no significant difference between bioaugmentation treated water
(BTW) and control (C). After 96 h, lipid peroxidation increased significantly in liver of catfish in
CPW group while there was no significant increase in liver of catfish in BTW group compared
with control. Reduced glutathione level, superoxide dismutase and catalase activity decreased
significantly in liver of catfish in CPW group with no significant difference found between BTW
group and control. These findings reveal that bioaugmentation technique is effective in overcoming
oxidative stress in fish exposed to crude oil even at lethal concentrations.
Keywords: Alcaligenes eutrophus; Bacillus sphaericus; bioaugmentation; catfish; Clarias
gariepinus; crude oil
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